Analysis of relationships between the protonmotive force and rates and extents of ATP synthesis.

The medium composition was as follows : 0.15 M-sucrose, 30rn~-KCl, 1OmM-choline chloride, 10mM-succinate/Tris, lOmM-Tris/Mops, 3mM-P,/Tris, 2pM-rotenone, 0.1 mMEGTA and 2mg of rat-liver mitochondria/ml; pH7.4. Temperature 25°C. Respiration was stimulated by 0.5 m ~ ADP ( 0 ) in the presence of 1 mM-EDTA, by 20pmol of FCCP/mg (0 ) or by 6Opmol of FCCP/mg (0). When the respiration was stimulated by ADP or by 6Opmol of FCCPlmg, malonate at a concentration between 0 and 5 m ~ was used as inhibitor. When respiration was stimulated by 20pmol of FCCP/mg the inhibitor was antimycin at a concentration between 0 and 12pmol/mg.